Journal of Siberian Federal University. Biology: Differentiation of South Siberian Populations of Corydalis bracteata Sensu Lato Based on the Data from Sequencing of Chloroplast DNA Sites, iPBS Markers, and Morphology of Underground Organs

Journal of Siberian Federal University. Biology / Differentiation of South Siberian Populations of Corydalis bracteata Sensu Lato Based on the Data from Sequencing of Chloroplast DNA Sites, iPBS Markers, and Morphology of Underground Organs

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Issue: Journal of Siberian Federal University. Biology. 2026 19 (2)
Authors: Ryabova, Kseniya K.; Yamskikh, Irina E.; Stepanov, Nikolay V.; Kutsev, Maksim G.
Contact information: Ryabova, Kseniya K.: Siberian Federal University (Krasnoyarsk, Russian Federation); Federal Research Center “Krasnoyarsk Science Center of the Siberian Branch of RAS” (Krasnoyarsk, Russian Federation); ; ORCID: 0009-0008-0896-4249; Yamskikh, Irina E. : Siberian Federal University (Krasnoyarsk, Russian Federation); ORCID: 0000-0003-1424-9547; Stepanov, Nikolay V. : Siberian Federal University (Krasnoyarsk, Russian Federation); ORCID: 0000-0002-0692-8796; Kutsev, Maksim G.: Altai State University (Barnaul, Russian Federation); ORCID: 0000-0003-2284-6851
Keywords: Corydalis bracteata; Corydalis talpina; Corydalis bombylina; Fumarioidae; Papaveraceae; atpB-rbcL; rpoB-trnC; rpl14-rps8-infA-rpl36; trnS-trnG-trnG; Corydalis bracteata; Corydalis talpina; Corydalis bombylina; Fumarioidae; iPBS markers; Papaveraceae; genetic diversity; atpB-rbcL; rpoB-trnC; rpl14-rps8-infA-rpl36; trnS-trnG-trnG
Abstract: This study examines the morphology of underground organs and the genetic diversity of Corydalis bracteata s. l. populations growing in plant communities of the Eastern Sayan, Western Sayan, Khamar-Daban, Kuznetsk Alatau, and Altai. Morphological analysis revealed the division of the populations into two groups. The presence of a large additional tuber in the axil of the basal scale and the plagiotropic orientation of the stolon bring the plants from the Eastern Sayan, Western Sayan, and Baikal populations closer to the recently described species Corydalis talpina Stepanov and C. bombylina Stepanov. The second group consists of C. bracteata populations growing in the Altai, Kuznetsk Alatau, and Western Sayan (Borus Range). This group is characterized by the absence of an additional tuber and an orthotropic stolon orientation. These results are supported by the iPBS analysis of the genetic variability of Corydalis populations. High values of the population subdivision coefficient (Gst = 0.3626 %) indicate the presence of different species within the total sample. The division into two groups was also demonstrated by constructing a similarity dendrogram and clustering genotypes using a Bayesian approach in the software STRUCTURE. Sequencing of chloroplast DNA (atpB-rbcL, rpoB-trnC, rpl14-rps8-infA-rpl36, trnS-trnG-trnG) from the type specimens of C. talpina and C. bombylina and the Altai specimen of C. bracteata revealed species-specific single- and di- nucleotide substitutions distinguishing these species. The congruent results of morphological and genetic analyses indicate differentiation among yellow-flowered Corydalis populations from Southern Siberia initially assigned to C. bracteata and point to the morphological and genetic distinctness of the group with an additional tuber. This conclusion is supported by the analysis of material from nine herbarium collections, which revealed that the Yenisei River serves as the boundary between the distributions of the two morphological forms of yellow-flowered Corydalis
Pages: 227–244
EDN: KEGTFH
Paper at repository of SibFU: https://elib.sfu-kras.ru/handle/2311/159243

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