Journal of Siberian Federal University. Biology / 3D Microscopy for the Evaluation of Laser Modification Outcomes in Degradable Polyhydroxyalkanoate Polymer Films

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Issue
Journal of Siberian Federal University. Biology. 2026 19 (2)
Authors
Dudaev, Aleksey E.; Turchin, Vladimir I.
Contact information
Dudaev, Aleksey E. : Institute of Biophysics SB RAS Federal Research Center “Krasnoyarsk Science Center SB RAS” (Krasnoyarsk, Russian Federation); Siberian Federal University (Krasnoyarsk, Russian Federation); ; ORCID: 0000-0003-4873-126X; Turchin, Vladimir I. : Siberian Federal University (Krasnoyarsk, Russian Federation)
Keywords
degradable polyhydroxyalkanoates; films; laser irradiation; microrelief; 3D microscopy
Abstract

Laser modification of biodegradable polyhydroxyalkanoate (PHA) films is a promising approach to tailoring their surface properties for biomedical applications. However, the quantitative relationship between polymer composition and the resulting 3D surface topography remains poorly understood. In this study, we use high-resolution 3D microscopy to evaluate the effects of CO2 laser treatment – under both continuous and quasi-pulsed modes – on films composed of four distinct PHAs: the homopolymer poly(3-hydroxybutyrate) [P(3HB)] and three copolymers – poly(3-hydroxybutyrate-co-3-hydroxyvalerate) [P(3HB-co-3HV)], poly(3-hydroxybutyrate- co-4-hydroxybutyrate) [P(3HB-co-4HB)], and poly(3- hydroxybutyrate-co-3-hydroxyhexanoate) [P(3HB-co-3HHx)]. Non-destructive 3D topographic mapping enabled precise quantification of groove and crater depths and uniformity. The homopolymer P(3HB) formed shallow (26.5 μm), fragmented structures, while copolymers, especially P(3HB-co-3HHx), produced deeper (up to 45.1 μm) and more uniform features, correlating with lower crystallinity and melting points. This study establishes 3D microscopy as an essential tool for quantitatively linking PHA composition to laser-induced microarchitecture, facilitating a rational design of degradable films with controlled topography

Pages
325–332
EDN
UQBXES
Paper at repository of SibFU
https://elib.sfu-kras.ru/handle/2311/159249

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