Journal of Siberian Federal University. Biology / Development of an Aptamer-Based Lateral Flow Biosensor for Rapid Detection of Cardiaс Troponin I

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Issue
Journal of Siberian Federal University. Biology. 2026 19 (2)
Authors
Krasitskaya, Vasilisa V.; Morozova, Elisaveta P.; Lukyanenko, Anna V.; Frank, Ludmila A.
Contact information
Krasitskaya, Vasilisa V.: Institute of Biophysics SB RAS Federal Research Center “Krasnoyarsk Science Center SB RAS” (Krasnoyarsk, Russian Federation); Siberian Federal University (Krasnoyarsk, Russian Federation); ORCID: 0000-0001-7018-2581; Morozova, Elisaveta P.: Institute of Biophysics SB RAS Federal Research Center “Krasnoyarsk Science Center SB RAS” (Krasnoyarsk, Russian Federation); Siberian Federal University (Krasnoyarsk, Russian Federation); Lukyanenko, Anna V. : Kirensky Institute of Physics Federal Research Center KSC SB RAS (Krasnoyarsk, Russian Federation); OCRID: 0000-0001-7496-242X; Frank, Ludmila A.: Institute of Biophysics SB RAS Federal Research Center “Krasnoyarsk Science Center SB RAS” (Krasnoyarsk, Russian Federation); Siberian Federal University (Krasnoyarsk, Russian Federation); ORCID: 0000-0003-4462-1944
Keywords
rapid diagnostics; cardiac troponin I; aptamers; gold nanoparticles; lateral analysis
Abstract

Coronary heart disease, heart attacks, and strokes are the most common and dangerous diseases that increase the mortality rate of a population. In Russia, cardiovascular diseases are the leading cause of death among people aged from 25 to 64 years. These diseases are often asymptomatic and, therefore, timely and reliable diagnostics is crucial for the success of therapy. The development of highly sensitive and specific analytical systems suitable for point- of-care (POC) use, regardless of the availability of a special room, equipment or specially trained personnel, is an urgent task. POC diagnostic devices should be cost- effective, portable, easy to use, and disposable. Cardiac troponin I (cTnI, 24 kDa) is one of the early, highly specific markers, the appearance of which in the peripheral blood indicates cardiomyocyte injury during myocardial infarction. Immunoassay is routinely used to detect it. Aptamers are considered a promising alternative to immunoglobulins. They have comparable sensitivity and specificity but are free of such disadvantages as complexity of production, thermal instability, and variability between batches of immunoglobulins. Aptamers are produced by chemical synthesis; they are stable and easily modifiable. Previously, the authors of the present study produced a pair of anti- troponin I DNA aptamers that detected this marker with high sensitivity (40 pM, 1 ng/ ml). The aim of this research was to develop a version of lateral flow analysis based on DNA aptamer sensors, suitable for rapid detection of cardiac troponin I. As a result of the study, a lateral flow analysis was designed using gold nanoparticles as an aptamer carrier and a visual signal generator, and model experiments were carried out to detect troponin I. The sensitivity of the model system was 24 ng/ml, and additional studies are required to enhance the detection signal

Pages
368–384
EDN
GUBVJK
Paper at repository of SibFU
https://elib.sfu-kras.ru/handle/2311/159252

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