Journal of Siberian Federal University. Biology / Biological Value of Microbial Biomass Derived from Cupriavidus necator Cultivated on Fish Processing Waste Oil

Full text (.pdf)
Issue
Journal of Siberian Federal University. Biology. 2026 19 (2)
Authors
Zhila, Natalia O.; Kiselev, Evgeniy G.; Sapozhnikova, Kristina Yu.; Volkov, Vladimir V.; Volova, Tatiana G.
Contact information
Zhila, Natalia O.: Institute of Biophysics SB RAS Federal Research Center “Krasnoyarsk Science Center SB RAS” (Krasnoyarsk, Russian Federation); Siberian Federal University (Krasnoyarsk, Russian Federation); ; ORCID: 0000‑0002‑6256‑0025; Kiselev, Evgeniy G.: Institute of Biophysics SB RAS Federal Research Center “Krasnoyarsk Science Center SB RAS” (Krasnoyarsk, Russian Federation); Siberian Federal University (Krasnoyarsk, Russian Federation); ORCID: 0000 ‑0003‑4472‑7087; Sapozhnikova, Kristina Yu. : Institute of Biophysics SB RAS Federal Research Center “Krasnoyarsk Science Center SB RAS” (Krasnoyarsk, Russian Federation); Siberian Federal University (Krasnoyarsk, Russian Federation); ORCID: 0000 ‑0001‑6054‑4200; Volkov, Vladimir V. : Kaliningrad State Technical University (Kaliningrad, Russian Federation); ORCID: 0000‑0001‑5560‑7131; Volova, Tatiana G. : Institute of Biophysics SB RAS Federal Research Center “Krasnoyarsk Science Center SB RAS” (Krasnoyarsk, Russian Federation); Siberian Federal University (Krasnoyarsk, Russian Federation); ORCID: 0000‑0001‑9392‑156X
Keywords
microbiological synthesis; fish processing waste; microbial biomass; single‑cell protein; amino acid score; digestibility
Abstract

The research aimed to determine biological value of microbial biomass synthesised by wild- type strains of Cupriavidus necator bacteria grown on oil derived from fish processing waste. This is the first reported synthesis of biomass samples by two strains of C. necator, B‑10646 and B‑15081, cultivated on oil extracted from the heads of Atlantic mackerel (Scomber scombrus), a waste stream obtained from the Department of Food Technology at Kaliningrad State Technical University. Cultivation was conducted under continuous mode in an automated Bio‑Flo 115 fermentation system at a dilution rate of 0.25 h-1. A preliminary assessment of the biological value of the resulting microbial biomass was performed, focusing on its suitability as single-cell protein (SCP). Key evaluation parameters included: (i) nutritional value (biochemical composition of the biomass, protein content and amino acid profile, including essential amino acids) and (ii) digestibility. The protein content in the biomass exceeded 60 % in both C. necator strains. The amino acid profiles of both strains were complete, containing all essential amino acids. Furthermore, the fractional composition of the protein – a critical indicator of its quality – was analysed. Fractions I and II, which are most susceptible to proteolytic degradation, constituted 41–42 % of the total protein, while Fractions III and IV accounted for about 55 %. In vitro digestibility assays using pepsin and trypsin confirmed the optimal distribution of protein fractions in the SCP from C. necator in comparison with reference animal and plant proteins – casein and wheat flour, respectively. Collectively, these findings demonstrate that the SCP produced by the examined strains on fish processing waste oil possesses high biological value. This novel, sustainable substrate enables the production of a high-quality SCP with strong potential for use as a protein supplement in livestock and aquaculture feeds

Pages
333–344
EDN
TZZIGO
Paper at repository of SibFU
https://elib.sfu-kras.ru/handle/2311/159250

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