Journal of Siberian Federal University. Biology / Effect of Zinc Deficiency and Excess on the Antioxidant Enzymes Activity in Barley Seedling Leaves

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Issue
Journal of Siberian Federal University. Biology. 2021 14 (3)
Authors
Kaznina, Natalya M.; Batova, Yulia V.; Repkina, Natalia S.
Contact information
Kaznina, Natalya M.: Institute of Biology Karelian Researches Centre RAS Petrozavodsk, Russian Federation; ; ORCID: 0000-0003-3092-563X; Batova, Yulia V.: Institute of Biology Karelian Researches Centre RAS Petrozavodsk, Russian Federation; ORCID: 0000-0003-3804-8651; Repkina, Natalia S.: Institute of Biology Karelian Researches Centre RAS Petrozavodsk, Russian Federation; ORCID: 0000-0002-8555-4701
Keywords
Hordeum vulgare L.; zinc deficiency; zinc excess; antioxidant enzymes; genes expression; Hordeum vulgare L.
Abstract

Zinc (Zn) is an essential microelement in plant nutrition but its high concentrations can be toxic to plants. Either Zn deficiency or its excess negatively affects plant metabolism, in particular due to alterations in cellular redox balance and development of oxidative stress. However, little is known about the effect of Zn deficiency and excess on the activity of antioxidant enzymes and expression of genes encoding them. The aim of this investigation was to study the effect of Zn deficiency and its excess on the intensity of oxidative processes, superoxide dismutase (SOD) and peroxidase (PO) activity, and genes (HvCu/ZnSOD1 and HvPRX07) expression in barley leaves (Hordeum vulgare L. cv. Nur). Plants were grown for 7 days at optimal zinc concentration (2 μM), its deficiency (0 μM) and excess (1000 μM). Both stress factors caused similar shoot growth inhibition. However, they both differently influenced the intensity of lipid peroxidation (LPO), total enzyme activity and gene expression. Zn deficiency led to an increase in mRNA content of HvPRX07 gene, while the activities of PO and SOD were lower compared to those at optimal Zn level. The LPO intensity did not increase. Zn excess caused a significant increase in HvCu/ZnSOD1 gene expression, and the activity of both enzymes. LPO intensity also increased. This may suggest that under zinc deficiency the inhibition of plant growth is not directly related to the changes of cell redox balance, whereas Zn excess results in an oxidative stress that can cause inhibition of shoot growth

Pages
287–295
DOI
10.17516/1997-1389-0351
Paper at repository of SibFU
https://elib.sfu-kras.ru/handle/2311/144318

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