Siberian Federal University 
- Issue
- Journal of Siberian Federal University. Chemistry. 2023 16 (3)
- Authors
- Kiselev, Evgeniy G.; Demidenko, Aleksey V.; Baranovskiy, Sergey V.; Volova, Tatiana G.
- Contact information
- Kiselev, Evgeniy G.: Institute of Biophysics SB RAS Krasnoyarsk, Russian Federation; Siberian Federal University Krasnoyarsk, Russian Federation; evgeniygek@gmail.com; Demidenko, Aleksey V.: Institute of Biophysics SB RAS Krasnoyarsk, Russian Federation; Siberian Federal University Krasnoyarsk, Russian Federation; Baranovskiy, Sergey V.: Siberian Federal University Krasnoyarsk, Russian Federation; Volova, Tatiana G.: Institute of Biophysics SB RAS Krasnoyarsk, Russian Federation; Siberian Federal University Krasnoyarsk, Russian Federation
- Keywords
- polyhydroxyalkanoates; poly‑3-hydroxybutyrate; solubilization; extraction; bacterial biomass; extraction technology
- Abstract
The process of isolating and purifying poly‑3-hydroxybutyrate under scaling conditions has been studied. Sodium dodecyl sulfate, sodium hypochlorite and Pemos washing powder were studied as solubilizing agents. The highest yield of 95 % and polymer purity of 99.5 % was achieved using a two- stage extraction method and Pemos powder as a solubilizing agent. As a result, it was possible to reduce the costs at the solubilization stage to 31 rubles/kg of poly‑3-hydroxybutyrate. Based on the research results, a technology for the isolation and purification of polyhydroxyalkanoates was proposed
- Pages
- 438–446
- EDN
- DPMYRV
- Paper at repository of SibFU
- https://elib.sfu-kras.ru/handle/2311/151720
Journal of Siberian Federal University. Chemistry / Isolation and Purification of Polyhydroxyalkanoates. Scaling in Pilot Production
Full text (.pdf)