Journal of Siberian Federal University. Biology / Waste Fish Oil is a Promising Substrate for the Synthesis of Target Products of Biotechnology

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Issue
Journal of Siberian Federal University. Biology. 2023 16 (3)
Authors
Zhila, Natalia O.; Volkov, Vladimir V.; Mezenova, Olga Ya.; Kiselev, Evgeniy G.; Volova, Tatiana G.
Contact information
Zhila, Natalia O.: Institute of Biophysics SB RAS, FRC “Krasnoyarsk Science Center SB RAS”; ; ORCID: 0000-0002-6256-0025; Volkov, Vladimir V.: Kaliningrad State Technical University Kaliningrad, Russian Federation; ORCID: 0000-0001-5560-7131; Mezenova, Olga Ya.: Kaliningrad State Technical University Kaliningrad, Russian Federation; Kiselev, Evgeniy G.: Institute of Biophysics SB RAS Krasnoyarsk, Russian Federation; Siberian Federal University Krasnoyarsk, Russian Federation; ; ORCID: 0000-0003-4472-7087; Volova, Tatiana G.: Institute of Biophysics SB RAS Krasnoyarsk, Russian Federation; Siberian Federal University Krasnoyarsk, Russian Federation; ORCID: 0000-0001-9392-156X
Keywords
waste fish oil; carbon substrate; biosynthesis; single cell protein; biodegradable bioplastics
Abstract

Fat derived from the waste of the Baltic sprat (Sprattus sprattus) canning industry was studied as a carbon substrate for the synthesis of single cell protein and degradable bioplastics, polyhydroxyalkanoates (PHAs), in the culture of three bacterial strains: Cupriavidus necator B‑5786, C. necator B‑8562, and C. necator B‑10646. The fatty substrate used in the present study contained 95 % of total lipids, 4 % of proteins, and 1 % of carbohydrates. Sixteen fatty acids (FAs) of lipids were identified, with palmitic (28.0 %), oleic (25.3 %), and docosahexaenoic (16.7 %) acids prevailing. The modes of cell cultivation were varied and the concentration of nitrogen in the medium was changed to direct metabolism towards synthesis of single cell protein or reserve PHAs. On complete nutrient medium, all strains synthesized high- protein biomass containing at least 70 and 50 % of “crude” protein and protein, respectively, which were complete in amino acids, including essential ones. When bacterial growth was limited by nitrogen, high (up to 60–70 %) yields of PHAs were obtained. The PHAs were represented by 3-component copolymers poly(3-hydroxybutyrtae-co‑3-hydroxyvalerate-co‑3-hydroxyhaxanoate) (P(3HB‑co‑3HV‑co‑3HHx)) with 0.20–0.31 mol.% of 3HV and 0.04–0.07 mol.% of 3HHx and with a weight average molecular weight of at least 600 kDa and a degree of crystallinity of about 70 %. Based on these parameters, the fat- containing waste of the canning industry can be regarded as a promising renewable substrate for the biotechnological production of single cell protein and biodegradable “green” plastics – polyhydroxyalkanoates

Pages
386–397
EDN
HORLYA
Paper at repository of SibFU
https://elib.sfu-kras.ru/handle/2311/151781

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