Siberian Federal University

Non-peptide selective blockers of AT1-receptors have been developed relatively recently and are actively used in thrombosis therapy. These substances belong to the group of drugs modulating the functioning of the renin-angiotensin-aldosterone system through interaction with angiotensin receptors. However, with a variety of methods for controlling this substance, the problem of quantitative determination of telmisartan in biological fluids remains relevant. Since the substance has a high fluorescent activity, an assumption has been formed on the possibility of developing a method of determining the amount of telmisartan by fluorescent method. The method has high sensitivity and selectivity that allows to determine fluorescence in solutions with concentration from 10–12 M. In this work fluorescent properties of telmisartan substance and “Telmista” medicine were obtained, analytical signal registration conditions were defined empirically: solvent – 0.01 M NaOH, excitation wave length – 290 nm, luminescence wave length – 365–490 nm, strobe parameters – signal delay 0.70 μs, signal duration – 4.70 μs. On the basis of the obtained spectra the calibration dependence of the signal intensity on the substance concentration was constructed, and the relative quantum yield was calculated. The influence of solvents in different pH conditions on the intensity of the analytical signal in the model solution was obtained. It was found that the highest intensity of luminescence was observed in 0.01 M NaOH. Quantitative determination of the drug “Telmista” in the model solution was carried out by fluorimetric method. The conditions of signal detection in human blood plasma were determined. This method is characterized by high selectivity, accuracy and sensitivity. The low level of spectral interference allows detection of weak analytical signals. It was found that the excipients meglumine, povidone- K30, lactose monohydrate, sorbitol (E 420), magnesium stearate, included in the drug “Telmista” have no interfering effect on the signal intensity, since the absorption spectra of these excipients are in the region of 500 nm [1: 40]. Fluorimetric analysis of telmisartan often uses a selected combination of chemical reaction products called EDAS (ethylenediamine, diacetylmonoxime, sodium sulfite) reagent. The reaction produces a commodity homogeneous mixture that absorbs light of a specific wavelength and emits light in precisely the range that allows the concentration of telmisartan to be accurately measured. Due to its increased sensitivity, the fluorimetric method of analysis is a very useful tool for measuring telmisartan concentration in human plasma. Moreover, it is able to detect extremely small amounts of the drug substance, which makes it particularly useful for clinical trials and drug analysis. However, the express method of fluorimetric determination of telmisartan will speed up the process of obtaining patient tests many times over. In order to develop such a method, the obtained described in this article were carried out