Journal of Siberian Federal University. Biology / Induction of Somatic Embryogenesis in Siberian Dwarf Pine (Pinus pumila (Pall.) Regel) in vitro

Full text (.pdf)
Issue
Journal of Siberian Federal University. Biology. 2024 17 (2)
Authors
Sheveleva, Irina S.; Golovanova, Tamara I.; Tretyakova, Iraida N.
Contact information
Sheveleva, Irina S. : Siberian Federal University Krasnoyarsk, Russian Federation; ; Golovanova, Tamara I. : Siberian Federal University Krasnoyarsk, Russian Federation; Tretyakova, Iraida N.: V.N. Sukachev Institute of Forest SB RAS Krasnoyarsk, Russian Federation; ORCID: 0000-0002-2029-5163
Keywords
Pinus pumila; in vitro; Pinus pumila; in vitro; Siberian dwarf pine; callus; embryogenic cultures; somatic embryogenesis
Abstract

Induction and proliferation of embryogenic calluses are the key stages in large-scale reproduction of coniferous species by somatic embryogenesis and long-term storage of their embryos. However, there are some issues, such as low reproduction efficiency, loss of embryogenic ability, poor viability. To solve the problems of somatic embryogenesis in the Siberian dwarf pine (Pinus pumila (Pall.) Regel), we aimed to determine the best cultivation conditions by varying the concentrations and combinations of plant growth regulators in the culture medium for inducing embryogenic calluses at different stages of embryo development. Explant response dependence on the stage of its development and the hormonal composition of the nutrient medium was established and optimal conditions for induction of somatic embryogenesis in Siberian dwarf pine were determined. Zygotic embryos with a length of 1 mm (mature embryo) were shown to be the most promising material for induction of Pinus pumila callogenesis (85 %). Embryogenic calluses were formed more actively on the DCR medium with reduced concentrations of plant growth regulators, NAA (1 mg/l) and 6-BAP (0.5 and 1 mg/l). Calluses transplanted to fresh media of the same composition every 14 days maintained their viability and sustained their ability to intensively proliferate throughout the year

Pages
148–159
EDN
LVBAPM
Paper at repository of SibFU
https://elib.sfu-kras.ru/handle/2311/153198

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