Siberian Federal University

Issue

Journal of Siberian Federal University. Biology. 2024 17 (2)

Authors

Pyatina, Svetlana A.; Menzyanova, Natalia G.; Shishatskaya, Ekaterina I.

Contact information

Pyatina, Svetlana A. : Siberian Federal University Krasnoyarsk, Russian Federation; ; ORCID: 0000-0003-3930-6923; Menzyanova, Natalia G.: Siberian Federal University Krasnoyarsk, Russian Federation; Shishatskaya, Ekaterina I.: Siberian Federal University; Krasnoyarsk, Russian Federation ORCID: 0000-0001-7967-243X

Keywords

oxidative stress; malondialdehyde; carbonylated proteins; root apex; border cells

Abstract

Currently, the prospects for increasing the efficiency of various agricultural technologies are associated with the use of nanomaterials as nanopesticides, nanofertilizers, nanoadaptogens, and seed priming agents. The successful development of nanoagricultural technologies is hampered by insufficient data on the mechanisms through which the biological activity of nanomaterials is realized at various stages of development of the plant organism. Therefore, in the present work, the effects of carbon nanoparticles – fullerenes (C 60F) and nanodiamonds (ND) – on the germination activity of grains and the functional status of the roots of 2-day-old wheat seedlings were studied in hydroponic wheat culture. The priming effect was detected only for C 60P (5 μg/mL). Carbon nanoparticles did not stimulate root growth. In the ND (25 and 50 μg/mL) treatments, root growth was inhibited. An increase in the population of border cells in the root apex was detected only in the C 60F (50 μg/mL) treatment, where the increase in the number of border cells was accompanied by an increase in the protein content in the mucilage cap of the root apex. A decrease in the number of border cells without changes in the protein content in the apical mucilage cap was detected in the ND (50 μg/mL) and C 60F (25 μg/mL) treatments. Both types of nanoparticles caused a dose-dependent increase in the secretory activity of border cells. Carbon nanoparticles had dose- dependent pro- oxidant activity. ND (5 μg/mL) and C 60F (5 and 25 μg/mL) increased the content of carbonylated proteins in the roots of 2-day-old seedlings. An increase in malondialdehyde content was observed only in the ND (50 μg/mL) treatment. The detected dose-dependent effects of carbon nanoparticles are not associated with their penetration into cells (the sizes of nanoparticles significantly exceed the sizes of nanopores in the cell wall of wheat). It is assumed that the biological activity of C 60F and ND can be realized through the induction of oxidative stress in the cell wall and apoplast. Results of the current study suggest that carbon nanoparticles (of certain size classes in certain concentrations) can be regarded as promising regulators of the physiology of the root system, realizing their activity “extracellularly”, without translocation into the cell

Pages

160–176

EDN

JOHDBL

Paper at repository of SibFU

https://elib.sfu-kras.ru/handle/2311/153199

This work is licensed under a Creative Commons Attribution-NonCommercial 4.0 International License (CC BY-NC 4.0).