Journal of Siberian Federal University. Biology / Effect of Exogenous Salicylic Acid on the Superoxide Dismutase Activity in Cucumber Seedlings (Cucumis sativus L.) Under Chilling

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Issue
Journal of Siberian Federal University. Biology. 2024 17 (2)
Authors
Repkina, Natalia S.; Ignatenko, Anna A.; Talanova, Vera V.
Contact information
Repkina, Natalia S. : Institute of Biology, Karelian Research Centre RAS Petrozavodsk, Russian Federation; ; ORCID: 0000‑0002‑8555‑4701; Ignatenko, Anna A.: Institute of Biology, Karelian Research Centre RAS Petrozavodsk, Russian Federation; Talanova, Vera V. : Institute of Biology, Karelian Research Centre RAS Petrozavodsk, Russian Federation
Keywords
Cucumis sativus; antioxidants; low temperature; phytohormone; gene expression; Cucumis sativus
Abstract

Phytohormones play a key role in adaptation to stress factors, including low temperature. There are data indicating a protective effect of salicylic acid (SA), a phenolic compound, on plants under chilling. Previously, it was shown that SA application had a positive effect on antioxidant enzyme activity but less data are available about changes in expression of genes encoding antioxidant enzymes. For this reason, the effects of exogenous SA on the superoxide dismutase (SOD) activity and Cu/Zn-SOD and Mn-SOD gene expression in cucumber (Cucumis sativus L.) seedlings under chilling stress (12 °C and 4 °C) were investigated. An analysis of electrolyte leakage from leaf cells and malondialdehyde (MDA) content showed that cucumber seedlings could survive at the temperature of 12 °C, but the temperature of 4 °C caused a significant damage. SOD activity in leaves increased gradually for 3 days at 12 °C, while at 4 °C, it increased during the first 5 h of exposure with a further decrease. The temperature of 12 °C induced accumulation of Cu/Zn-SOD and Mn-SOD gene transcripts in leaves, whereas 4 °C did not affect gene expression. Exogenous salicylic acid (SA) (100 μM) application reduced the level of electrolyte leakage and MDA concentration but increased SOD activity and Cu/Zn-SOD and Mn-SOD gene expression at both 12 °C and 4 °C. It is suggested, that the protective role of SA under the chilling stress is associated with its involvement in the regulation of the antioxidant system of plants, particularly the activity of SOD and expression of genes encoding SOD isoforms

Pages
177–189
EDN
VWZMMZ
Paper at repository of SibFU
https://elib.sfu-kras.ru/handle/2311/153200

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