Journal of Siberian Federal University. Biology / Fatty Acids Composition in Cyanobacteria and Their Ability to Synthesize Degradable Polyhydroxyalkanoates (PHA)

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Issue
Journal of Siberian Federal University. Biology. 2024 17 (3)
Authors
Zhila, Natalia O.; Kalacheva, Galina S.; Shishatskaya, Ekaterina I.; Ghorabe, Fares D. E.; Volova, Tatiana G.
Contact information
Zhila, Natalia O. : Institute of Biophysics SB RAS Federal Research Center “Krasnoyarsk Science Center SB RAS” Krasnoyarsk, Russian Federation; ; ORCID: 0000-0002-6256-0025; Kalacheva, Galina S.: Institute of Biophysics SB RAS Federal Research Center “Krasnoyarsk Science Center SB RAS” Krasnoyarsk, Russian Federation; Shishatskaya, Ekaterina I.: Infochemistry Scientific Centre, ITMO University Saint Petersburg, Russian Federation; ORCID: 0000-0001-7967-243X; Ghorabe, Fares D. E.: Infochemistry Scientific Centre, ITMO University Saint Petersburg, Russian Federation; Volova, Tatiana G.: Institute of Biophysics SB RAS Federal Research Center “Krasnoyarsk Science Center SB RAS” Krasnoyarsk, Russian Federation; ORCID: 0000-0001-9392-156X
Keywords
cyanobacteria; water bodies of the Yenisei River Basin; fatty acid composition of lipids; synthesis of polyhydroxyalkanoates
Abstract

The composition of fatty acids of lipids and the ability of cyanobacteria to synthesize degradable polyhydroxyalkanoates (PHA) were examined in previously unstudied cyanobacteria isolated from a number of water bodies of the Yenisei River Basin. Ten strains of various species belonging to the orders Chroococcales, Oscillatoriales, Nostocales and Leptolyngbyales were investigated. Thirty-three fatty acids (FAs) were identified in the lipid composition of the studied cyanobacterial strains. The ratio of FAs was to a high extent species-specific with the lipid saturation ranging from 0,4 to 9,5. In all strains, palmitic acid was identified as the main FA with the content ranging from 24 to 47 % of the total FA. The dominant monoenoic FAs were C16:1ɷ7 and C18:1ɷ9; their content varied from 2 to 16 and from 2 to 24 % of the total FA, respectively. All strains synthesized polyenoic acids of the C16- and C18 series but in different amounts. Five strains (Chroococcus limneticus ACCS019, Trichormus variabilis ACCS039, Trichormus variabilis ACCS060, Anabaena aequalis ACCS089 and Cylindrospermum stagnale ACCS051) were determined to be able to synthesize a homopolymer of 3-hydroxybutyric acid – poly(3-hydroxybutyrate). A modification of the standard nutrient medium (the exclusion of nitrogen and phosphorus from its composition) ensured a multiple increase in the intracellular content of the polymer. The cyanobacteria Chroococcus limneticus ACCS019, Anabaena aequalis ACCS089 and Cylindrospermum stagnale ACCS051 with the polymer content of 8.9, 4.7 and 3.2 %, respectively, can be considered as potential producers of PHA in photosynthesis

Pages
264–277
EDN
LTFCNM
Paper at repository of SibFU
https://elib.sfu-kras.ru/handle/2311/153846

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