Journal of Siberian Federal University. Biology / Development and Characterization of Novel DNA Aptamers Specific to Heart-Type Fatty Acid Binding Protein (hFABP)

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Issue
Journal of Siberian Federal University. Biology. 2024 17 (3)
Authors
Krasitskaya, Vasilisa V.; Vereshchagina, Tatiana A.; Smoliarova, Tatiana E.; Sokolov, Alexey E.; Tupikin, Alexey E.; Dominguez-Arca, Vicente; Frank, Ludmila A.
Contact information
Krasitskaya, Vasilisa V. : Institute of Biophysics SB RAS Federal Research Center “Krasnoyarsk Science Center SB RAS” Krasnoyarsk, Russian Federation; ORCID: 0000-0001-7018-2581; Vereshchagina, Tatiana A. : Institute of Chemistry and Chemical Technology Federal Research Center “Krasnoyarsk Science Center SB RAS” Krasnoyarsk, Russian Federation; ORCID: 0000-0003-4538-8902; Smoliarova, Tatiana E.: Kirensky Institute of Physics Federal Research Center “Krasnoyarsk Science Center SB RAS” Krasnoyarsk, Russian Federation; ORCID: 0000-0002-0123-1965; Sokolov, Alexey E.: Kirensky Institute of Physics Federal Research Center “Krasnoyarsk Science Center SB RAS” Krasnoyarsk, Russian Federation; Tupikin, Alexey E.: Institute of Chemical Biology and Fundamental Medicine SB RAS Novosibirsk, Russian Federation; ORCID: 0000-0002-8194-0322; Dominguez-Arca, Vicente: University of Santiago de Compostela Santiago de Compostela, Spain; ORCID: 0000-0002-3500-5915; Frank, Ludmila A. : nstitute of Biophysics SB RAS Federal Research Center “Krasnoyarsk Science Center SB RAS” Krasnoyarsk, Russian Federation; ; ORCID: 0000-0003-4462-1944
Keywords
DNA aptamers; heart-type fatty acid-binding protein (hFABP); Ca2+-regulated photoprotein obelin; isothermal titration calorimetry (ITC)
Abstract

Heart-type fatty acid-binding protein (hFABP) has been proposed as a new biochemical marker for the early diagnosis of acute myocardial infarction (AMI). The work describes the development of high-affinity and specific DNA aptamers as sensor elements of analytical systems for the rapid detection of this marker. Several novel DNA aptamers to hFABP were selected by using hFABP-activated magnetic microparticles as a target. The DNA library enrichment, affinity and specificity of candidate aptamers as well their truncated variants, were examined by solid-phase obelin-based bioluminescent assay. High binding ability was shown for the aptamer FABPAp4 by applying isothermal titration calorimetry (ITC) technique. The developed aptamers suggest to contain G-quadruplex (GQ) forming motifs that play a key role in binding the target. Demonstrated high affinity and specificity for hFABP determine the prospects of the obtained aptamers as sensor elements of analytical systems intended for AMI early diagnosis

Pages
302–319
EDN
GTBCMR
Paper at repository of SibFU
https://elib.sfu-kras.ru/handle/2311/153849

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