Siberian Federal University 
- Issue
- Journal of Siberian Federal University. Mathematics & Physics. 2024 17 (6)
- Authors
- Panamarev, Nikita S.; Tyumentseva, Anna V.; Krasitskaya, Vasilisa V.; Frank, Ludmila A.
- Contact information
- Panamarev, Nikita S.: Institute of Biophysics SB RAS, Federal Research Center KSC SB RA Krasnoyarsk, Russian Federation; OCRID: 0000-0001-6954-7280; Tyumentseva, Anna V.: Krasnoyarsk Scientific Center SB RAS Krasnoyarsk, Russian Federation; OCRID: 0000-0002-2269-0298; Krasitskaya, Vasilisa V.: Institute of Biophysics SB RAS, Federal Research Center KSC SB RAS Krasnoyarsk, Russian Federation; OCRID: 0000-0001-7018-2581; Frank, Ludmila A.: Institute of Biophysics SB RAS, Federal Research Center KSC SB RAS Krasnoyarsk, Russian Federation; OCRID: 0000-0003-4462-1944
- Keywords
- DNA aptamers; survivin (BIRC5); magnetic nanoparticles
- Abstract
Magnetic iron oxide nanoparticles modified with aminosiloxane ( -Fe2O3@SiO2-NH2) were used as a carrier to select DNA aptamers for a small protein tumor biomarker survivin (BIRC5, 16.5 kDa) from a randomized synthetic DNA library by the SELEX method. Survivin was covalently immobilized on the nanoparticles’ surface. The library enrichment was monitored by solid-phase bioluminescent immunoassay and the DNA melting method. The prospects are outlined for the use of the obtained nanoparticles for the effective selection of aptamers specific to a given target
- Pages
- 769–775
- EDN
- UYVQZC
- Paper at repository of SibFU
- https://elib.sfu-kras.ru/handle/2311/154115
Journal of Siberian Federal University. Mathematics & Physics / Magnetic Particles as a Carrier to Select Aptamer for Survivin (BIRC5), a Low Molecular Weight Protein Tumor Marker
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