Journal of Siberian Federal University. Mathematics & Physics / Structure Determination of the Pb2+-specific GT Aptamer Using SAXS and Molecular Modeling Methods

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Issue
Journal of Siberian Federal University. Mathematics & Physics. 2024 17 (6)
Authors
Artyushenko, Polina V.; Shchugoreva, Irina A.; Berlina, Anna N.; Komova, Nadezhda S.; Serebrennikova, Kseniya V.; Moryachkov, Roman V.; Zabluda, Vladimir N.; Sokolov, Alexey E.; Tomilin, Felix N.; Kichkailo, Anna S.
Contact information
Artyushenko, Polina V. : Federal Research Center KSC SB RAS Krasnoyarsk, Russian Federation; Prof. V. F. Voino-Yasenetsky Krasnoyarsk State Medical University Krasnoyarsk, Russian Federation; Shchugoreva, Irina A. : Federal Research Center KSC SB RAS Krasnoyarsk, Russian Federation; Prof. V. F. Voino-Yasenetsky Krasnoyarsk State Medical University Krasnoyarsk, Russian Federation; OCRID: 0000-0003-4207-1627; Berlina, Anna N. : A. N. Bach Institute of Biochemistry, Research Center of Biotechnology RAS Moscow, Russian Federation; ; Komova, Nadezhda S. : A. N. Bach Institute of Biochemistry, Research Center of Biotechnology RAS Moscow, Russian Federation; ; Serebrennikova, Kseniya V. : A. N. Bach Institute of Biochemistry, Research Center of Biotechnology RAS Moscow, Russian Federation; ; Moryachkov, Roman V. : Kirensky Institute of Physics, Federal Research Center KSC SB RAS Krasnoyarsk, Russian Federation; OCRID: 0000-0002-0409-779X; Zabluda, Vladimir N. : Kirensky Institute of Physics, Federal Research Center KSC SB RAS Krasnoyarsk, Russian Federation; ; Sokolov, Alexey E. : Kirensky Institute of Physics, Federal Research Center KSC SB RAS Krasnoyarsk, Russian Federation; Siberian Federal University Krasnoyarsk, Russian Federation; ; Tomilin, Felix N. : Federal Research Center KSC SB RAS Krasnoyarsk, Russian Federation; Kirensky Institute of Physics, Federal Research Center KSC SB RAS Krasnoyarsk, Russian Federation; Siberian Federal University Krasnoyarsk, Russian Federation; ; Kichkailo, Anna S. : Federal Research Center KSC SB RAS Krasnoyarsk, Russian Federation; Prof. V. F. Voino-Yasenetsky Krasnoyarsk State Medical University Krasnoyarsk, Russian Federation; OCRID: 0000-0003-0690-7837
Keywords
aptamers; heavy metal ions; small-angle X-ray scattering; circular dichroism spectroscopy; molecular modeling
Abstract

Aptamer GT conjugated with gold nanoparticles is specific for heavy metal ions and can be used for water quality control and environmental monitoring. Circular dichroism (CD) and small- angle X-ray scattering (SAXS) measurements were performed to determine the structure of the aptamer complex with lead (II) ions. Comparison of the measurement results with different options of the spatial structure of the complexes obtained using molecular modeling allowed us to determine how the aptamer changes its conformation due to interacting with the target

Pages
808–816
EDN
VTQVOG
Paper at repository of SibFU
https://elib.sfu-kras.ru/handle/2311/154141