Journal of Siberian Federal University. Mathematics & Physics / Design of Aptamer Sandwich Pairs for Colorimetric Detection of TNFa, Interleukin-17A and Dickkopf-1 Proteins Associated with Ankylosing Spondylitis

Full text (.pdf)
Issue
Journal of Siberian Federal University. Mathematics & Physics. 2024 17 (6)
Authors
Shatunova, Elizaveta A.; Davydova, Anna S.; Danilin, Nikolay A.; Golyshkin, Arseniy A.; Korolev, Maksim A.; Vorobyeva, Mariya A.
Contact information
Shatunova, Elizaveta A. : Institute of Chemical Biology and Fundamental Medicine SD RAS Novosibirsk, Russian Federation; ; Davydova, Anna S. : Institute of Chemical Biology and Fundamental Medicine SD RAS Novosibirsk, Russian Federation; ; Danilin, Nikolay A.: Institute of Chemical Biology and Fundamental Medicine SD RAS Novosibirsk, Russian Federation; ; Golyshkin, Arseniy A. : Institute of Chemical Biology and Fundamental Medicine SD RAS Novosibirsk, Russian Federation; ; Korolev, Maksim A. : Institute of Chemical Biology and Fundamental Medicine SD RAS Novosibirsk, Russian Federation; Research Institute of Clinical and Experimental Lymphology Affiliated Branch of Federal Research Center of Cytology and Genetics SD RAS Novosibirsk, Russian Federation; ; Vorobyeva, Mariya A. : Institute of Chemical Biology and Fundamental Medicine SD RAS Novosibirsk, Russian Federation; OCRID: 0000-0001-5317-3288
Keywords
oligonucleotide aptamers; biosensors; colorimetric detection; ankylosing spondylitis
Abstract

We employed DNA and 2’-fluoro RNA aptamers to assembly novel systems for colorimetric microplate detection of TNFa, interleukin-17A and Dickkopf-1 proteins as biomarkers associated with ankylosing spondylitis. For each protein, we chose an optimal sandwich pair made of plate-immobilized capture aptamer and biotinylated reporter aptamer recruiting streptavidin-peroxidase conjugate. A feasibility of these test systems was demonstrated in model protein solutions

Pages
791–797
EDN
VKMXNM
Paper at repository of SibFU
https://elib.sfu-kras.ru/handle/2311/154144