Siberian Federal University

Three-dimensional cell culture is a modern tool for cancer research. This cell-based model combines the simplicity and accessibility of two-dimensional systems with mimicking in vivo conditions via supporting cell- cell and cell-matrix interactions. This allows it to be used both for studying the mechanisms of cancerogenesis and for preclinical testing anticancer therapeutics. In this research, we propose a simple and detailed technique for the preparation of spheroids composed of human glioblastoma cells embedded in a biopolymer matrix based on modified pectins supplemented with collagen I. The protocol also includes long-term cell monitoring with comprehensive analysis of cell viability, migration and behavior in culture using conventional optical imaging, laser scanning microscopy and flow cytometry