Siberian Federal University

Issue

Journal of Siberian Federal University. Biology. 2024 17 (4)

Authors

Permyakova, Marina D.; Permyakov, Alexey V.

Contact information

Permyakova, Marina D. : Siberian Institute of Plant Physiology and Biochemistry SB RAS Irkutsk, Russian Federation; ; ORCID: 0000-0002-0259-8531; Permyakov, Alexey V. : Siberian Institute of Plant Physiology and Biochemistry SB RAS Irkutsk, Russian Federation; ORCID: 0000-0003-0002-5470

Keywords

Aegilops tauschii Coss.; lipoxygenase; wheat; Aegilops tauschii Coss.; introgression; water deficit; stress adaptation

Abstract

Lipoxygenase (LOX), a key enzyme of lipid metabolism, is involved in signaling pathways of adaptation to an unfavorable environment. Introgression of wild cereal genes that control LOX activity can be useful in breeding wheat for tolerance to stress, including water deficit. Using Western blotting, we compared the isozyme composition of LOX in the leaves of the synthetic hexaploid ‘Synthetic 6x’ (Syn6x), carrying the D genome from Aegilops tauschii Coss., and the wheat ‘Chinese Spring’ (CS). Four forms of lipoxygenase, different in intracellular localization, were studied: soluble, membrane-bound (without chloroplast membranes), soluble chloroplast and chloroplast membrane- bound. The studies were carried out under optimal water supply and simulated soil drought. Five soluble and six membrane- bound molecular forms of LOX have been found. Sin6x, as compare to CS, had two additional molecular forms of soluble and membrane LOX but it lacked three chloroplast LOX isoforms found in CS. Water deficit induced two molecular forms of LOX: soluble in Syn6x and bound to chloroplast membranes in CS. Spectrometry was used to study LOX activity in CS, Syn6x and twenty introgressive recombinant lines which carried different sites of introgression from Ae. tauschii in the 2D chromosome of the CS genome. In most lines, introgression of wild cereals caused an increase in enzymatic activity. Under water deficit, Syn6x increased the activity of soluble LOX, while CS increased the activity of chloroplast forms of the enzyme. In introgressive lines, the patterns of LOX activity changes were similar to one of the parental patterns (CS or Syn6x) for each of the four forms of LOX studied

Pages

409–419

EDN

JPHRZI

Paper at repository of SibFU

https://elib.sfu-kras.ru/handle/2311/154338

This work is licensed under a Creative Commons Attribution-NonCommercial 4.0 International License (CC BY-NC 4.0).