Siberian Federal University

Issue

Journal of Siberian Federal University. Biology. 2025 18 (1)

Authors

Zaytseva, Tatyana B.; Kuzikova, Irina L.; Medvedeva, Nadezda G.

Contact information

Zaytseva, Tatyana B.: St. Petersburg Federal Research Center of the Russian Academy of Sciences St. Petersburg, Russian Federation; ; Kuzikova, Irina L.: St. Petersburg Federal Research Center of the Russian Academy of Sciences St. Petersburg, Russian Federation; ORCID: 0000-0001-6199-3104; Medvedeva, Nadezda G.: St. Petersburg Federal Research Center of the Russian Academy of Sciences St. Petersburg, Russian Federation; ORCID: 0000-0003-0588-8427

Keywords

Lelliottia jeotgali 18s; nonylphenol; octylphenol; Lelliottia jeotgali 18s; aquatic and soil ecosystems; biodegradation; bioaugmentation; soil enzymatic activity

Abstract

Alkylphenols (AP) are the most extensively employed chemicals in industry and agriculture. Because of their large-scale application, AP are detected in soil and aquatic environments. The hazard posed by alkylphenols is associated with their ability to affect negatively the functioning of the endocrine system of living organisms. The currently used strategies for remediation of soils and water contaminated with AP are based on chemical or physical treatment technologies. However, these methods are either ineffective or too expensive. Modern technologies of recovering soil and water properties, which are based on bioremediation involving microorganisms degrading harmful substances, are more effective. The purpose of this research was to isolate and identify a bacterial strain that degrades nonyl- and octylphenols; to study the kinetics of AP degradation by the isolated strain; to assess the dynamics of changes in the enzymatic activity of AP‑contaminated soil bio-augmented with a contaminant-degrading bacterial strain. AP‑degrading bacteria were isolated from soil using the enrichment culture method. Identification of the AP‑degrading bacterial strain was performed using methods based on phenotypic characteristics of bacteria and the sequencing method. The AP degradation process in the soil and water samples was detected by high-performance liquid chromatography. Strain 18s, which is capable of degrading alkylphenols, was isolated from the soil sample treated with nonylphenol. To identify the strain, the 16S rRNA gene sequencing methods were used, and its morphological, cultural, physiological, and biochemical traits were taken into account. The strain was identified as Lelliottia jeotgali 18s. The study showed that the strain L. jeotgali 18s was able to degrade long-chain alkylphenols – nonyl- and octylphenols – both in water and in soil. Soil augmentation with L. jeotgali 18s accelerated the recovery of enzymatic activity in soil contaminated with alkylphenols. The results obtained in the current work suggest that L. jeotgali 18s needs to be studied further as a promising strain for the development of methods of biological treatment of natural ecosystems to remove endocrine disruptors

Pages

5–21

EDN

UHCCPT

Paper at repository of SibFU

https://elib.sfu-kras.ru/handle/2311/155061

This work is licensed under a Creative Commons Attribution-NonCommercial 4.0 International License (CC BY-NC 4.0).