Siberian Federal University 
- Issue
- Journal of Siberian Federal University. Chemistry. 2014 7 (4)
- Authors
- Okunev, Rodion V.; Grigoryan, Boris R.; Sharipova, Adelina I.
- Contact information
- Okunev, Rodion V.:Kazan (Volga region) Federal University 18 Kremlyovskaya str., Kazan, 420008, Russia; E-mail: ; Grigoryan, Boris R.:Kazan (Volga region) Federal University 18 Kremlyovskaya str., Kazan, 420008, Russia; Sharipova, Adelina I.:Kazan (Volga region) Federal University 18 Kremlyovskaya str., Kazan, 420008, Russia
- Keywords
- amino acid derivatization; soil solution; free amino acids; amino acids in soil; phenyl isothiocyanate; HPLC
- Abstract
A high-performance liquid chromatography method for the determination of seventeen free proteinogenic amino acids (FPAA) in soil solutions is described. Sample preparation involves obtaining soil solution and pre-column derivatization of free amino acids using phenyl isothiocyanate. Phenyl thiocarbamyl derivatives are separated on a reversed-phase column by using linear gradient elution with 140 mM sodium acetate buffer solution (pH 6.0) contained 0.05% triethylamine as mobile phase A and acetonitrile-water (60:40,v/v) as mobile phase B. The described method showed adequate linearity, recovery values, limits of detection, and suitable for the determination of FPAA in soil solutions
- Pages
- 480-486
- Paper at repository of SibFU
- https://elib.sfu-kras.ru/handle/2311/16592
Journal of Siberian Federal University. Chemistry / Determination of Free Proteinogenic Amino Acids in Soil Solutions by HPLC with Phenyl Isothiocyanate Derivatization
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