Study of Biocompatible Films and Nonwoven Membranes Made of Copolymer of 3-hydroxybutyric Acid and 4-hydroxybutyric Acid in vitro

Full text (.pdf)
Issue
Биология. Biology. 2016 9 (1)
Authors
Borovkova, Natalia V.; Evseev, Anatoly K.; Makarov, Maksim S.; Goroncharovskaya, Irina V.; Vinogradova, Olga N.; Nikolaeva, Elena D.; Goncharov, Dmitry B.
Contact information
Borovkova, Natalia V.:N.V. Sklifosovsky Institute of Emergency Care 3 Bolshaya Suharevskaya sq,. Moscow, 129090, Russia; Evseev, Anatoly K.:N.V. Sklifosovsky Institute of Emergency Care 3 Bolshaya Suharevskaya sq,. Moscow, 129090, Russia; Makarov, Maksim S.:N.V. Sklifosovsky Institute of Emergency Care 3 Bolshaya Suharevskaya sq,. Moscow, 129090, Russia; Goroncharovskaya, Irina V.:N.V. Sklifosovsky Institute of Emergency Care 3 Bolshaya Suharevskaya sq,. Moscow, 129090, Russia; Vinogradova, Olga N.:Siberian Federal University 79 Svobodny, Krasnoyarsk, 660049, Russia; Nikolaeva, Elena D.:Siberian Federal University 79 Svobodny, Krasnoyarsk, 660049, Russia; E-mail: ; Goncharov, Dmitry B.:Siberian Federal University 79 Svobodny, Krasnoyarsk, 660049, Russia
Keywords
polyhydroxyalkanoates; copolymers P(3HB/4HB); production; characteristics; fibroblasts cultivation; wound dressing
Abstract

Results of studies of films and nonwoven membranes made of a copolymer of 3-hydroxybutiric and 4-hydroxybutyric acid [P(3HB/4HB)] as a models for wound dressings are presented. Structure and properties of P(3HB/4HB) product surface and mechanical properties depending on production method and ultrafine fibers orientation of nonwoven membranes obtained by electrospinning are studied. It is shown in cultures of mouse fibroblasts NIH 3T3 and diploid cells of human embryonic lines M-22 all the investigated P(3HB/4HB) samples are biocompatible, not cytotoxic in direct contact with cells and provide high adhesion and cell proliferation, as well as maintaining a high physiological activity up to 7 days. The P(3HB/4HB) film samples and the nonwoven membrane are suitable as biotransplantats and wound coverings

Pages
43-52
Paper at repository of SibFU
http://elib.sfu-kras.ru/handle/2311/20180