Differentiation of Mscs into Osteoblasts on a Porous 3D -Carrier of Poly-3-Hydroxybutyrate

Full text (.pdf)
Issue
Биология. Biology. 2016 9 (1)
Authors
Shumilova, Anna A.; Nikolaeva, Elena D.
Contact information
Shumilova, Anna A.:Institute of Biophysics of SB RAS 50/50 Akademgorodok, Krasnoyarsk, 660036, Russia; E-mail: ; Nikolaeva, Elena D.:Institute of Biophysics of SB RAS 50/50 Akademgorodok, Krasnoyarsk, 660036, Russia; Siberian Federal University 79 Svobodny, Krasnoyarsk, 660041, Russia
Keywords
cellular carrier; poly-3-hydroxybutyrate; mesenchymal stem cells; differentiation; osteoblasts
Abstract

The study describes preparation and testing of porous 3D implants of natural degradable polymer of 3-hydroxybutyric acid [P(3HB)] for regeneration of bone tissue defects. The ability of the 3D carriers to favor attachment and facilitate proliferation and directed differentiation into osteoblasts of mesenchymal stem cells (MSCs) was studied in the culture of MSCs isolated from bone marrow and adipose tissue. MSC differentiation into osteoblasts was confirmed by measurements of the activity of alkaline phosphatase, gene expression of the marker of bone protein BGP (glutamic protein participating in mineralization) and measurements of intracellular precipitates of calcium and phosphorus salts

Pages
53-62
Paper at repository of SibFU
http://elib.sfu-kras.ru/handle/2311/20181

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