Properties and Biocompatibility of Poly-3-Hydroxybutyrate-co-3-Hydroxyvalerate/ Poly-ε-Caprolactone Blends

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Биология. Biology. 2016 9 (1)
Boyandin, Anatoly N.; Nikolaeva, Elena D.; Sukovatiy, Aleksey G.
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Boyandin, Anatoly N.:Institute of Biophysics of SB RAS 50/50 Akademgorodok, Krasnoyarsk, 660036, Russia; Siberian Federal University 79 Svobodny, Krasnoyarsk, 660041, Russia; E-mail: ; Nikolaeva, Elena D.:Institute of Biophysics of SB RAS 50/50 Akademgorodok, Krasnoyarsk, 660036, Russia; Sukovatiy, Aleksey G.:Institute of Biophysics of SB RAS 50/50 Akademgorodok, Krasnoyarsk, 660036, Russia
poly-3-hydroxybutyrate-co-3-hydroxyvalerate copolymer; poly-ε-caprolactone; polymer blends; biopolymers; biocompatibility

Blends consisting of the poly-3-hydroxybutyrate-co-3-hydroxyvalerate (PHBV) copolymer and poly- ε-caprolactone (PCL) were used to prepare polymer films by casting solution technique. The structure and physical-mechanical, and biological properties of the films were examined as influenced by the ratios of the blend components. The microstructure of the films was determined by the dominant component: the films had porous surface if the percentage of PHBV was higher than that of PCL and were relatively smooth if the dominant component was PCL. The highest elongation at break (190-368 %) was exhibited by the films prepared from the blends containing 5-25 % PHBV; at 25 % PHBV, local maxima of tensile strength (16 MPa) and Young’s modulus (529 MPa) were observed. Biocompatibility of the films prepared from the blends was studied in the culture of NIH 3T3 mouse fibroblast cells. Results of SEM and DAPI staining, determination of the number of attached cells, and MTT assay showed the absence of cytotoxic effects and high cell adhesion and proliferation, which in some cases were higher than on films prepared from pure PHBV and PCL. Thus, PHBV/PCL blends are suitable materials for constructing cell scaffolds for tissue engineering and other reconstructive technologies

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