Siberian Federal University

Issue

Journal of Siberian Federal University. Biology. 2011 4 (3)

Authors

Zhila, Natalia O.; Kalachova, Galina S.; Volova, Tatiana G.

Contact information

Zhila, Natalia O. : Institute of Biophysics Siberian Branch of Russian Academy of Science Siberian Federal University , 50 Akademgorodok, Krasnoyarsk, 660036 Russia 79 Svobodny, Krasnoyarsk, 660041 Russia , e-mail: ; Kalachova, Galina S. : Institute of Biophysics Siberian Branch of Russian Academy of Science , 50 Akademgorodok, Krasnoyarsk, 660036 Russia; Volova, Tatiana G. : Institute of Biophysics Siberian Branch of Russian Academy of Science Siberian Federal University , 50 Akademgorodok, Krasnoyarsk, 660036 Russia 79 Svobodny, Krasnoyarsk, 660041 Russia

Keywords

Botryococcus; Botryococcus; biochemical composition; fatty acids; hydrocarbons; salinity

Abstract

The effect of 0,3 and 0,7 M NaCl on biomass yield, total nitrogen content, intracellular lipid content, hydrocarbons and fatty acid profile of the lipids of the alga Botryococcus braunii Kütz IPPAS H-252 in different phases of the culture cycle was studied. The presence of sodium chloride in the medium inhibited the growth of algal cells for the first three days of the experiment, causing a decrease in total nitrogen, enhanced synthesis of triacylglycerols. In addition, considerable changes in the lipid fatty acid profile were found, i.e. decrease in polyunsaturated fatty acid (PUFA) levels (29,38 % and 12,8 %) and levels of long-chain saturated acids (5,3 % and 14,13 % of the total) at 0,3 M NaCl and 0,7 M NaCl, respectively. In later phases of the culture, at 0,3 M NaCl, the PUFA content increased up to the values characteristic of the active growth phase of the alga. At 0,7 M NaCl, the proportion of PUFA enhanced at less extent, but biomass concentration and total nitrogen increased, similarly to the experiment with 0,3 M NaCl, that may also be indicative of adaptation of the alga to the studied concentrations of NaCl.

Pages

229-242

Paper at repository of SibFU

https://elib.sfu-kras.ru/handle/2311/2724

This work is licensed under a Creative Commons Attribution-NonCommercial 4.0 International License (CC BY-NC 4.0).