Siberian Federal University

Issue

Journal of Siberian Federal University. Biology. 2012 5 (3)

Authors

Volova, Tatiana G.; Shishatskaya, Ekaterina I.; Zhila, Natalia O.; Kiselev, Eugeny G.; Mironov, Petr V.; Vasiliev, Alexander D.; Peterson, Ivan V.; Sinskey, Anthony J.

Contact information

Volova, Tatiana G. : Institute of Biophysics of SB RAS Siberian Federal University , Akademgorodok, Krasnoyarsk 660036, Russia 79 Svobodny, Krasnoyarsk 660041, Russia , e-mail: ; Shishatskaya, Ekaterina I. : Institute of Biophysics of SB RAS Siberian Federal University , Akademgorodok, Krasnoyarsk 660036, Russia 79 Svobodny, Krasnoyarsk 660041, Russia; Zhila, Natalia O. : Institute of Biophysics of SB RAS Siberian Federal University , Akademgorodok, Krasnoyarsk 660036, Russia 79 Svobodny, Krasnoyarsk 660041, Russia; Kiselev, Eugeny G. : Institute of Biophysics of SB RAS Siberian Federal University , Akademgorodok, Krasnoyarsk 660036, Russia 79 Svobodny, Krasnoyarsk 660041, Russia; Mironov, Petr V. : Siberian State Technology University , 82 Mira av., Krasnoyarsk, Russia; Vasiliev, Alexander D. : Institute of Physics named after L.V.Kirenskii SB RAS , Akademgorodok, Krasnoyarsk 660036, Russia; Peterson, Ivan V. : Institute of Chemistry and Chemical Technology SB RAS , Akademgorodok, Krasnoyarsk 660036, Russia; Sinskey, Anthony J. : Siberian Federal University Massachusetts Institute of Technology , 79 Svobodny, Krasnoyarsk 660041, Russia Cambridge, MA 02139, USA

Keywords

biopolymers; polyhydroxyalkanoates; substrates; synthesis; properties; processing; biomedical applications

Abstract

Microbial polyhydroxyalkanoates (PHAs) have long been studied due to their potential for replacement of petroleum-based plastic. The study addresses the effect of different conditions of carbon nutrition on synthesis of polyhydroxyalkanoates by the bacterium Ralstonia eutropha. First and foremost, PHAs are carbon storage compounds for many organisms. There are still many aspects of the physiology of PHA accumulation and degradation that are still not understood. Measurements of key P3HBrelated enzyme activities throughout cell growth reveal correlations of acetoacetyl-CoA reductase and synthase enzyme activity maxima with P3HB biosynthesis. The investigation addressed kinetic parameters of growth and accumulation of polyhydroxyalkanoates and gas exchange parameters of the culture of the CO-resistant strain of the hydrogen bacteria Ralstonia eutropha B 5786 cultivated on synthesis gas - a product of gasification of brown coals. The results were compared with those obtained by growing the bacteria on electrolytic hydrogen and it was concluded that synthesis gas can be successfully used to produce PHAs. In experiments with wild-type strain it has been first found that under mixotrophic growth conditions - CO2 + co-substrate (alcanoic acids) - bacteria can synthesize multi-component PHAs, consisting of short- and medium-chain-length monomers with carbon chains containing 4 to 8 atoms. It has been shown that PHA composition is determined by the type of the co-substrate. Fatty acids with odd number of carbons induce bacteria to synthesize multi-component PHAs with 3-hydroxybutyrate, 3-hydroxyvalerate, and 3-hydroxyhexanoate as major monomers and 3-hydroxyheptanoate and 3-hydroxyoctanoate as minor, occasionally occurring, ones. Fatty acids with even number of carbons induce synthesis of not only their respective monomers (3-hydroxyhexanoate and 3-hydroxyoctanoate) but also 3-hydroxyvalerate, making possible synthesis of four-component PHAs, containing 3-hydroxybutyrate and 3-hydroxyhexanoate as major components. A family of short- and medium-chain-length four- and five-component PHAs has been synthesized and their physicochemical and biomedical-properties examined.

Pages

280-299

Paper at repository of SibFU

https://elib.sfu-kras.ru/handle/2311/3157

This work is licensed under a Creative Commons Attribution-NonCommercial 4.0 International License (CC BY-NC 4.0).