Journal of Siberian Federal University. Biology / Formation of Callus and Induction of Somatic Embryos Culture in Du Tour

Full text (.pdf)
Issue
Journal of Siberian Federal University. Biology. 2013 6 (1)
Authors
Tretyakova, Iraida N.; Voroshilova, Elena V.; Shuvaev, Denis N.; Lukina, Anastasya S.
Contact information
Tretyakova, Iraida N.: V.N. Sukachev Institute of Forest SB RAS, 50/28 Akademgorodok, Krasnoyarsk, 660036 Russia; e-mail: ; Voroshilova, Elena V.: Siberian Federal University, 79 Svobodny, Krasnoyarsk, 660041 Russia;; Shuvaev, Denis N.: Siberian Federal University, 79 Svobodny, Krasnoyarsk, 660041 Russia;; Lukina, Anastasya S.: V.N. Sukachev Institute of Forest SB RAS, 50/28 Akademgorodok, Krasnoyarsk, 660036 Russia;
Keywords
Siberian pine; pollen of annual cedar; zygotic embryos; hybrid embryos; callus; embryogenic mass; somatic embryos
Abstract

In culture in vitro nonembriogenic callus was inducted from immature zygotic embryos of seeds of Siberian pine trees (Pinus sibirica), growing in natural stands of West Sayan and in a clonal plantation. The active callus development goes in explants hybrid seeds, getting as results of control pollination of clones by pollen of Siberian pine trees with annual cycle development of female cones. Cytological study shows that the strong elongation in hypocotyl cells goes at the stage of callus induction. Only explants of embryo seeds of some clones, which were pollination of pollen trees-accelerate reached the stage of proliferation and formed embrygenic mass. Globules of somatic embryos and embryonal tubes are developed from embryonal initiales.

Pages
44-60
Paper at repository of SibFU
https://elib.sfu-kras.ru/handle/2311/9689

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