Siberian Federal University

Issue

Journal of Siberian Federal University. Biology. 2013 6 (2)

Authors

Shesternya, Pavel A.; Sergeeva, Anna S.; Nikulina, Svetlana Yu.; Shulman, Vladimir A.

Contact information

Шестерня, П.А.:Красноярский государственный медицинский университет им. В.Ф. Войно-Ясенецкого Россия 660022, Красноярск, ул. Партизана Железняка, 1; E-mail: ; Сергеева, А.С.:Красноярский государственный медицинский университет им. В.Ф. Войно-Ясенецкого Россия 660022, Красноярск, ул. Партизана Железняка, 1; Никулина, С.Ю.:Красноярский государственный медицинский университет им. В.Ф. Войно-Ясенецкого Россия 660022, Красноярск, ул. Партизана Железняка, 1; Шульман, В.А.:Красноярский государственный медицинский университет им. В.Ф. Войно-Ясенецкого Россия 660022, Красноярск, ул. Партизана Железняка, 1; Shesternya, Pavel A.:Krasnoyarsk State Medical University named after Рrof. V.F. Voino-Yasenetsky, 1 Partizan Zheleznyak Str., Krasnoyarsk, 660022 Russia; Sergeeva, Anna S.:Krasnoyarsk State Medical University named after Рrof. V.F. Voino-Yasenetsky, 1 Partizan Zheleznyak Str., Krasnoyarsk, 660022 Russia; Nikulina, Svetlana Yu.:Krasnoyarsk State Medical University named after Рrof. V.F. Voino-Yasenetsky, 1 Partizan Zheleznyak Str., Krasnoyarsk, 660022 Russia; Shulman, Vladimir A.:Krasnoyarsk State Medical University named after Рrof. V.F. Voino-Yasenetsky, 1 Partizan Zheleznyak Str., Krasnoyarsk, 660022 Russia

Keywords

rs10757278, rs1333049; myocardial infarction; single nucleotide polymorphisms; rs10757278, rs1333049; locus 9p21.3, atherosclerosis

Abstract

The purpose of this study was to investigate association between 9p21.3 locus single nucleotide polymorphisms (SNP) and severity of coronary atheromatous burden. A total of 255 Caucasians (211 male, 44 female) with myocardial infarction younger 65 years old (mean 52,56±7,98 years) were enrolled. Genome DNA was extracted from venous blood using the phenol-chloroform extraction method. Two SNPs rs10757278 and rs1333049 (locus 9p21.3) were tested using real-time polymerase chain reaction (PCR) according to protocol (probes TaqMan, Applied Biosystems, 7900HT). The coronary angiograms were reviewed by independent angiographers who were blinded to the results of the genotype (Philips Allura Xper FD10). The total number of lesions, Gensini scores and SYNTAX score were derived. For the first time in males of Siberian population, genotype CC rs1333049 and genotype GG rs10757278 demonstrated a direct strong association with severity of coronary atheromatous burden

Pages

175-184

Paper at repository of SibFU

https://elib.sfu-kras.ru/handle/2311/9982

This work is licensed under a Creative Commons Attribution-NonCommercial 4.0 International License (CC BY-NC 4.0).