Journal of Siberian Federal University. Biology / Fatty Acid Content and Composition of Fly Larvae Lucilia sericata (Family Calliphoridae) Grown on Diets with Different Content of Polyunsaturated Fatty Acids and the Amino Acid Composition of this Species

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Issue
Journal of Siberian Federal University. Biology. 2022 15 (3)
Authors
Stoyanov, Kirill N.; Makhutova, Olesia N.; Malyshevsky, Konstantin G.; Borisova, Elena V.; Sushchik, Nadezhda N.; Kolmakova, Anzhelika A.; Morgun, Vasiliy N.; Gladyshev, Michail I.
Contact information
Stoyanov, Kirill N.: Institute of Biophysics SB RAS FRC “Krasnoyarsk Science Center SB RAS” Krasnoyarsk, Russian Federation; Siberian Federal University Krasnoyarsk, Russian Federation; ; ORCID: 0000-0002-6983-771X; Makhutova, Olesia N.: Institute of Biophysics SB RAS FRC “Krasnoyarsk Science Center SB RAS” Krasnoyarsk, Russian Federation; Siberian Federal University Krasnoyarsk, Russian Federation; ORCID: 0000-0002-9387-5054; Malyshevsky, Konstantin G.: Institute of Biophysics SB RAS FRC “Krasnoyarsk Science Center SB RAS” Krasnoyarsk, Russian Federation; Borisova, Elena V.: Siberian Federal University Krasnoyarsk, Russian Federation; Sushchik, Nadezhda N.: Institute of Biophysics SB RAS FRC “Krasnoyarsk Science Center SB RAS” Krasnoyarsk, Russian Federation; Siberian Federal University Krasnoyarsk, Russian Federation; ORCID: 0000-0001-5587-9807; Kolmakova, Anzhelika A.: Institute of Biophysics SB RAS Federal Research Center “Krasnoyarsk Science Center SB RAS” Krasnoyarsk, Russian Federation; ORCID: 0000-0003-4926-1212; Morgun, Vasiliy N.: Siberian Federal University Krasnoyarsk, Russian Federation; Gladyshev, Michail I.: Institute of Biophysics SB RAS FRC “Krasnoyarsk Science Center SB RAS” Krasnoyarsk, Russian Federation; Siberian Federal University Krasnoyarsk, Russian Federation; ORCID: 0000-0003-2276-3095
Keywords
polyunsaturated fatty acids; alpha-linolenic fatty acid; fishmeal; terrestrial insects; fish oil; aquaculture; amino acids
Abstract

Aquaculture is a fast-growing branch of agriculture, but it faces fish feed shortages due to a decrease in wild fish catches. As a result, the price of feed increases. For further development it requires alternative feed sources. Insects are considered a suitable protein source for fish, but their fatty acid (FA) composition often does not meet the requirements of aquaculture. In fish oil, PUFAs are dominated by the omega‑3 family, and in terrestrial insects, by the omega‑6 family. A question arises whether insect larvae lipid composition can be modified to increase the content of omega‑3 PUFAs. For this purpose, Lucilia sericata larvae were grown on standard feed and feed with addition of camelina oil rich in alpha-linolenic acid (ALA, 18:3n‑3), and their FA content and composition were compared. To evaluate the quality of these larvae protein, their amino acid (AA) composition was determined. The FA analysis was performed on a gas chromatograph equipped with a mass-spectrometer detector. The AA analysis was performed on a liquid chromatograph. The AA composition of the examined fly larvae, similarly to other insects (Diptera), was close to the AA composition of fish meal. Fatty acid composition and content of fly larvae grown on standard food was characterized by a low ratio of omega‑3/omega‑6 PUFAs and by the dominance of 18:1n‑9 and 18:2n‑6 fatty acids, which together comprised 40–60 % of the total of FAs. The addition of camelina oil changed the ratio of omega‑3/ omega‑6 PUFAs from 0.11 to 0.46, mainly due to the increase in ALA content. Thus, FA content and composition of L. sericata larvae can be significantly modified by a diet

Pages
378–395
DOI
10.17516/1997-1389-0394
Paper at repository of SibFU
https://elib.sfu-kras.ru/handle/2311/149184

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