Siberian Federal University

Issue

Journal of Siberian Federal University. Biology. 2023 16 (2)

Authors

Baimukhametova, Elvina A.; Shvets, Darya Yu.; Musin, Khalit G.; Kuluev, Bulat R.

Contact information

Baimukhametova, Elvina A.: Institute of Biochemistry and Genetics, IBG UFRC RAS Ufa, Russian Federation; ; ORCID: 0000-0003-3147-915X; Shvets, Darya Yu.: Institute of Biochemistry and Genetics, IBG UFRC RAS Ufa, Russian Federation; Bashkir State Medical University Ufa, Russian Federation; ORCID: 0000-0003-4292-4562; Musin, Khalit G.: Institute of Biochemistry and Genetics, IBG UFRC RAS Ufa, Russian Federation; ORCID: 0000-0001-7336-2027; Kuluev, Bulat R.: Institute of Biochemistry and Genetics, IBG UFRC RAS Ufa, Russian Federation; Ufa University of Science and Technology Ufa, Russian Federation; ORCID: 0000-0002-1564-164X

Keywords

Agrobacterium rhizogenes; hairy roots; Agrobacterium rhizogenes; Agrobacterium-mediated transformation

Abstract

Chicory (Cichorium intybus L.) is a perennial herbaceous plant, a promising source of inulin in functional nutrition. Inulin is a polysaccharide consisting of fructofuranose residues. Its traditional production is associated with a number of difficulties, such as the need for thorough cleaning of the roots and dependence on climatic conditions, but these can be overcome by means of biotechnology. As biotechnological production of inulin is currently non-existent in Russia, obtaining cultures of chicory hairy roots capable of producing inulin is of paramount value. It can provide an opportunity for launching biotechnological production of inulin in Russia in the future. In this study, twelve lines of hairy root culture of C. intybus ‘Kofeek’ and wild type plants were obtained and the content of inulin in them was quantified. For this purpose, leaf explants obtained from in vitro plants were subjected to transformation with the A4 strain Agrobacterium rhizogenes containing the binary vector pCambia 1301. The content of inulin was determined by the spectrophotometric method. The inulin content in hairy root culture of wild type chicory plants and chicory ‘Kofeek’ averaged 1.46±0.46 % and 1.34±0.34 %, respectively, while the native roots of wild chicory contained 13.13±1.9 % inulin, and ‘Kofeek’ 11.55±2.32 %. The results indicate that the content of inulin in hairy roots is significantly lower than in native ones. Obtaining inulin from hairy roots under conditions of industrial cultivation in bioreactors may have a number of advantages, such as independence of weather conditions, no need for preliminary cleaning of the roots, and the possibility of year-round cultivation, which may partially compensate for the low content of inulin. However, further research aimed at increasing inulin content in hairy root culture is of immense importance

Pages

218–231

EDN

RBDTRY

Paper at repository of SibFU

https://elib.sfu-kras.ru/handle/2311/150840

This work is licensed under a Creative Commons Attribution-NonCommercial 4.0 International License (CC BY-NC 4.0).