Siberian Federal University

Manilkara zapota, an evergreen tree with multiple varieties, is cultivated for its delectable fruit. Different parts of the tree have been used in traditional medicine to cure a wide range of ailments, including fever, dysentery, and diarrhea. Owing of this tree's medicinal potential, several portions of it have been the subject of investigation. Manilkara zapota (L.) seed oil extraction and purification were examined in this work. Standard techniques were used to evaluate the seed oil's physico- chemical characteristics. FT-IR was used to determine the presence of functional groups in alkane, carbonyl, alkene, and methyl ester. The oil's primary chemical constituents were also examined using 1H and 13C NMR spectroscopy. Utilizing GC–MS spectroscopy, the chemical composition of the oil extracted from M. Zapota seed was determined. The activities of oil against bacteria and fungi were also evaluated. Both Gram-positive and Gram-negative bacteria were resistant to the oil. It has a strong reaction to S. typhi while showing no reaction to E. coli. When the oil's antifungal activity was evaluated against Aspergillus Niger and Aspergillus flavum, excellent inhibitory results were obtained. The derived oil has significant antibacterial and antifungal properties, according to this study