Siberian Federal University 
- Issue
- Journal of Siberian Federal University. Biology. Prepublication
- Authors
- Sapozhnikova, Kristina Yu.
- Contact information
- Sapozhnikova, Kristina Yu. : Institute of Biophysics SB RAS Federal Research Center “Krasnoyarsk Science Center SB RAS” Krasnoyarsk, Russian Federation; Siberian Federal University Krasnoyarsk, Russian Federation; ; ORCID: 0000-0001-6054-4200
- Keywords
- Cupriavidus necator; polyhydroxyalkanoates; bioplastics; low-grade animal fat; fatty acids; copolymers; Cupriavidus necator
- Abstract
Low-grade animal fats (lamb, beef, pork fats) – the large-tonnage waste of the food industry – were studied for the first time for the synthesis of degradable polyhydroxyalkanoates (PHAs). The composition of fatty acids in fats and the effect on the parameters of a laboratory batch culture of the wild-type strain Cupriavidus necator B-10646 were characterized. An effective process in terms of the total concentration of bacterial biomass grown in glass flasks was obtained on lamb fat (3.3±0.2 g/L); on beef fat, the biomass concentration was slightly lower (2.4±0.3 g/L) but polymer yields were high (58–64 %). Low values of these parameters were obtained on the most saturated fat – pork fat (1.2±0.3 g/L and 12 %, respectively). More technologically advanced three-component copolymers containing 3-hydroxybutyrate monomers as the major component (over 96 mol.%) and 3-hydroxyvalerate and 3-hydroxyhexanoate monomers as the minor ones (2.5–3.3 and 0.4–0.9 mol.%, respectively) were synthesized on all fats. The results contribute to solving the problem of reducing the cost of PHA production and making these polymers more accessible by synthesizing them on waste materials, which corresponds to modern trends of transition from a linear to a circular economy
- EDN
- CXYPVA
- Paper at repository of SibFU
- https://elib.sfu-kras.ru/handle/2311/157483
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