Nanodiamonds in Biotechnology: Application for Purification of Proteins and Test-Systems of Indication

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Issue
Биология. Biology. 2010 3 (4)
Authors
Ronzhin, Nikita O.; Kharin, Konstantin A.; Puzyr, Alexey P.; Bondar, Vladimir S.
Contact information
Ronzhin, Nikita O. : Siberian Federal University , 79 Svobodny, Krasnoyarsk, 660041 Russia , e-mail: ; Kharin, Konstantin A. : Siberian Federal University , 79 Svobodny, Krasnoyarsk, 660041 Russia; Puzyr, Alexey P. : Institute of Biophysics SB RAS , 50/50 Akademgorodok, Krasnoyarsk, 660036 Russia; Bondar, Vladimir S. : Siberian Federal University Institute of Biophysics SB RAS , 79 Svobodny, Krasnoyarsk, 660041 Russia 50/50 Akademgorodok, Krasnoyarsk, 660036 Russia
Keywords
modified detonation nanodiamonds; recombinant luciferase; protein purification; biotechnology; bioluminescence; test-system
Abstract

This paper discusses the perspectives of applications of the detonation nanodiamonds in biotechnology. An effective separation of proteins from complex protein mixtures by using of nanodiamonds is shown on the example of purification of recombinant luciferase from the extracts of E. coli bacterial cells: the process requires minimum equipment and time; one technological cycle yields 40-45 % of desired product with high-quality (according to the SDS-PAGE). The application of nanodiamonds for design of indicating systems is demonstrated on the example of a biosensor system based on the nanodiamondluciferase complex and inert polymeric matrix.

Pages
418-433
Paper at repository of SibFU
http://elib.sfu-kras.ru/handle/2311/2249

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