Journal of Siberian Federal University. Biology / Thermoinactivation of Coupled Enzyme System of Luminous Bacteria NADH:FMN-Oxidoreductase-Luciferase in Gelatin

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Issue
Journal of Siberian Federal University. Biology. 2011 4 (1)
Authors
Bezrukikh, Anna E.; Esimbekova, Elena N.; Kratasyuk, Valentina A.
Contact information
Bezrukikh, Anna E. : Siberian Federal University , 41 Svobodny, Krasnoyarsk, 660041 Russia , e-mail: ; Esimbekova, Elena N. : Siberian Federal University Institute of Biophysics , 41 Svobodny, Krasnoyarsk, 660041 Russia 50/50 Akademgorodok, Krasnoyarsk, 660036 Russia; Kratasyuk, Valentina A. : Siberian Federal University Institute of Biophysics , 41 Svobodny, Krasnoyarsk, 660041 Russia 50/50 Akademgorodok, Krasnoyarsk, 660036 Russia
Keywords
bacterial luciferase; NADH:FMN-oxidoreductase; bioluminescence; thermoinactivation of enzymes; stabilization of enzymes; gelatin
Abstract

Influence of gelatin solution and gel on bioluminescence of coupled enzymatic system of luminous bacteria NADH:FMN-oxidoreductase-luciferase was analyzed, thermostability and thermoinactivation of coupled enzyme system in gelatinous environment were studied. Activity of coupled enzyme system and kinetics of its thermoinactivation in gelatin with low concentration, that is insufficient for gel formation, and in gelatin with high concentration, that is sufficient for gel formation, was different. It was determined that thermoinactivation of coupled enzyme system has nonlinear nature and occurs with dissociative mechanism. It was showed that gel matrix of gelatin stabilizes enzymes. There was no effect of viscous environment originating by gelatin surroundings on the rate of enzymatic reactions.

Pages
64-74
Paper at repository of SibFU
https://elib.sfu-kras.ru/handle/2311/2374

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