Журнал СФУ. Биология / Biocompatibility of Polyhydroxybutyrate Microspheres: in vitro and in vivo Evaluation

Полный текст (.pdf)
Номер
Журнал СФУ. Биология. 2008 1 (1)
Авторы
Shishatskaya, Ekaterina I.; Voinova, Olga N.; Goreva, Anastasya V.; Mogilnaya, Olga A.; Volova, Tatiana G.
Контактная информация
Shishatskaya, Ekaterina I.: Institute of Biophysics of Siberian Branch of Russian Academy of Sciences, Akademgorodok, Krasnoyarsk, 660036, Russia;; Voinova, Olga N.: Institute of Biophysics of Siberian Branch of Russian Academy of Sciences, Akademgorodok, Krasnoyarsk, 660036, Russia; Goreva, Anastasya V.: Institute of Biophysics of Siberian Branch of Russian Academy of Sciences, Akademgorodok, Krasnoyarsk, 660036, Russia; Mogilnaya, Olga A.: Institute of Biophysics of Siberian Branch of Russian Academy of Sciences, Akademgorodok, Krasnoyarsk, 660036, Russia; Volova, Tatiana G.: Institute of Biophysics of Siberian Branch of Russian Academy of Sciences, Akademgorodok, Krasnoyarsk, 660036, Russia; Siberian Federal University, Svobodny av.79, Krasnoyarsk, 660041, Russia
Ключевые слова
microencapsulation; resorbable polymers; polyhydroxybutyrate; cytotoxicity; tissue response
Аннотация

Microspheres have been prepared from the resorbable linear polyester of β-hydroxybutyric acid (polyhydroxybutyrate, PHB) by the solvent evaporation technique and investigated in vitro and in vivo. Biocompatibility of the microspheres has been proved in tests in the culture of mouse fibroblast cell line NIH 3Т3 and in experiments on intramuscular implantation of the microspheres to Wistar rats for 3 months. Tissue response to the implantation of polymeric microspheres has been found to consist in a mild inflammatory reaction, pronounced macrophage infiltration that increases over time, involving mono- and poly-nuclear foreign body giant cells that resorb the polymeric matrix. No fibrous capsules were formed around polymeric microparticles; neither necrosis nor any other adverse morphological changes and tissue transformation in response to the implantation of the PHB microparticles were recorded. The results of the study suggest that polyhydroxybutyrate is a good candidate for fabricating prolonged-action drugs in the form of microparticles intended for intramuscular injection.

Страницы
66-77
Статья в архиве электронных ресурсов СФУ
https://elib.sfu-kras.ru/handle/2311/635

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